Site: Home > Home > News and events

Methods for improving column efficiency in liquid chromatography

2024-02-26 14:29

To improve the efficiency of liquid chromatography, the following aspects can be taken into consideration.

(1) Reducing the flow rate of the moving phase will prolong the analysis time.

(2) Reduce the amount of stationary phase, but the sample loading in the chromatographic column also decreases accordingly.

(3) Reduce the particle size of the stationary phase, but not excessively, as excessive reduction can also reduce the permeability of the chromatographic column.

(4) Choosing a low viscosity mobile phase is beneficial for rapid mass transfer, but it is not conducive to multi-component analysis.

(5) Properly increasing the column temperature can reduce the viscosity of the mobile phase, but the column efficiency and separation degree also decrease accordingly.

(6) Minimize the volume of the stationary moving phase as much as possible, but accelerate the flow rate of the moving phase.

From the above introduction, it can be seen that various factors are interrelated and restrictive in the process of chromatographic analysis. Only by tracking and calculating the column efficiency value, continuously researching and practicing our own analytical methods, can we find the best working conditions.

Related News

2024-02-26Direction of liquid chromatography column
2024-02-26Methods for improving column efficiency in liquid chromatography
2024-02-26Improving the Resolution of Gas Chromatography
2024-02-26Pure water machine reverse osmosis membrane cleaning
2024-02-26Replacing the filter element of the pure water machine
2024-02-26RO reverse osmosis pure water machine
2024-02-26How to install a pure water machine
2024-02-26Pure water machine bucket cleaning process
2024-02-26Identification of the quality of hydraulic oil
2024-02-26Reasons for high column pressure in liquid chromatography

Copyright 2022:Qinsun Instruments Co., Limited

High-end textile tester supplier | Textile Testing Equipment pdf | Tel:021-67800179 |